Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets.

نویسندگان

  • Bo Zheng
  • L Spencer Roach
  • Rustem F Ismagilov
چکیده

Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins.

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عنوان ژورنال:
  • Journal of the American Chemical Society

دوره 125 37  شماره 

صفحات  -

تاریخ انتشار 2003